Biochemistry and Metabolism of Giardia

ABSTRACT Giardia lamblia , an aerotolerant anaerobe, respires in the presence of oxygen by a flavin, iron‐sulfur protein‐mediated electron transport system. Glucose appears to be the only sugar catabolized by the Embden‐Meyerhof‐Pamas and hexose monophosphate pathways, and energy is produced by substrate level phosphorylation. Substrates are incompletely oxidized to CO 2 , ethanol and acetate by nonsedimentable enzymes. The lack of incorporation of inosine, hypoxanthine, xanthine, formate or glycine into nucleotides indicates an absence of de novo purine synthesis. Only adenine, adenosine, guanine and guanosine are salvaged, and no interconversion of these purines was detected. Salvage of these purines and their nucleosides is accomplished by adenine phosphoribosyltransferase, adenosine hydrolase, guanosine phosphonbosyltransferase and guanine hydrolase. The absence of de novo pyrimidine synthesis was confirmed by the lack of incorporation of bicarbonate, orotate and aspartate into nucleotides, and by the lack of detectable levels of the enzymes of de novo pyrimidine synthesis. Salvage appears to be accomplished by the action of uracil phosphoribosyltransferase, uridine hydrolase, uridine phosphotransferase, cytidine deaminase, cytidine hydrolase, cytosine phosphoribosyltransferase and thymidine phosphotransferase. Nucleotides of uracil may be converted to nucleotides of cytosine by cytidine triphosphate synthetase, but thymidylate synthetase and dihydrofolate reductase activities were not detected. Uptake of pyrmidine nucleosides, and perhaps pyrimidines, appears to be accomplished by carrier‐mediated transport, and the common site for uptake of uridine and cytidine is distinct from the site for thymidine. Thymine does not appear to be incorporated into nucleotide pools. Giardia trophozoites appear to rely on preformed lipids rather than synthesizing them de novo. Major lipids include phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingomyelin, sterol (probably cholesterol) and mono‐, di‐ and triacylglycer‐ides. The lipid composition of the cysts of G. lamblia isolated from gerbils and G. muris isolated from mice are similar to those obtained from the trophozoites of G. lamblia grown in vitro. The activities of several hydrolases of G. lamblia have been shown to be confined to a single lysosome‐like particle population with an equilibrium density of approximately 1.15 in sucrose. Contrary to the trophozoites of Entamoeba and the trichomonads, Giardia trophozoites appear to lack most carbohydrate splitting hydrolases. Calmodulin has been reported in G. lamblia trophozoites, and it appears to have properties similar to the calmodulin isolated from other eucaryotic cells.