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Effects of Cold and Nocodazole Treatments on the Microtubular Systems of Paramecium in Interphase
Author(s) -
TORRES ANTONIO,
DELGADO PILAR
Publication year - 1989
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1989.tb01056.x
Subject(s) - nocodazole , microtubule , tubulin , acetylation , cytoplasm , biology , paramecium , microbiology and biotechnology , biophysics , cytoskeleton , biochemistry , cell , gene
The sensitivity of the microtubular systems to cold and nocodazole treatments as well as the cellular distribution of microtubules containing acetylated a‐tubulin have been studied in Paramecium. Our results indicate that the microtubular systems of Paramecium show marked differences in stability. Cold and nocodazole treatments produce the depolymerization of the major part of the cytoplasmic microtubular network while the cortical structures remain stable under treatment. This differential stability of the microtubular systems is associated with the acetylation of the a‐tubulin. The use of the antibody 6‐11B‐1, specific for acetylated α‐tubulin, shows that the microtubular systems containing acetylated a‐tubulin are the same as those that remain stable after depoly‐merizing treatments. Finally, the images obtained during the repolymerization by heating suggest that the cortical dikinetids are able to nucleate the microtubules of the cytoplasmic network.