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The Lost Neuromotor Apparatus of Chlamydomonas : Rediscovered 1 , 2
Author(s) -
Salisbury J. L.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb06128.x
Subject(s) - chlamydomonas , ultrastructure , electron microscope , resolution (logic) , biology , staining , immunofluorescence , biophysics , nucleus , physics , botany , optics , microbiology and biotechnology , biochemistry , antibody , computer science , genetics , artificial intelligence , gene , immunology , mutant
. Early light microscopic studies of the biflagellate green alga Chlamydomonas revealed a fibrous system, the neuromotor apparatus , which appeared to link physically the flagellar apparatus to the cell nucleus. Following the development of the electron microscope, the existence of a neuromotor apparatus in Chlamydomonas was cast into doubt since it was not observed in studies carried out at ultrastructural resolution. Here we show, by indirect immunofluorescence, using monoclonal antibodies and electorn microscopy employing refined specimen preparation and staining techniques, that the neuromotor apparatus of Chlamydomonas does indeed exist. The functional significance of this system is discussed in light of both historic proposals and recent experimental findings.
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