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Improved Method for High‐Yield Excystation and Purification of Infective Sporozoites of Eimeria spp. 1
Author(s) -
HOSEK JAMES E.,
TODD KENNETH S.,
KUHLENSCHMIDT MARK S.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04156.x
Subject(s) - eimeria , biology , parasite hosting , protozoa , apicomplexa , plasmodium (life cycle) , microbiology and biotechnology , virology , protozoal disease , malaria , immunology , computer science , world wide web
This report describes a new, gentle procedure for rapid and efficient excystation of large numbers of infective sporozoites of Eimeria vermiformis and Eimeria stiedai. Excysted sporozoites are purified using modifications of a previously described ion‐exchange chromatography method. The procedure avoids physical breakage of oocysts and results in greater than 70% recovery of the sporozoites present as sporulated oocysts (i.e. 5–6 sporozoites per sporulated oocyst). The recovered sporozoites are greater than 95% pure and are infective in vivo. We routinely isolate greater than 2 times 10 8 sporozoites without the use of specialized or expensive equipment.