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Demonstration of Acid Phosphatase in Eimeria spp.: Partial Characterization of the Enzyme in E. vermiformis 1 , 2
Author(s) -
HOSEK JAMES E.,
TODD KENNETH S.,
KUHLENSCHMIDT MARK S.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04145.x
Subject(s) - acid phosphatase , biology , enzyme , tartrate , enzyme assay , phosphatase , biochemistry , virulence , protozoa , tartrate resistant acid phosphatase , alkaline phosphatase , microbiology and biotechnology , gene
Sporozoite extracts of E. vermiformis, E. stiedai , and E. tenella are rich in acid phosphatase activity. They contain specific enzyme activities equal to or greater than those reported for other highly virulent protozoan parasites. The absolute amount of enzyme activity per oocyst dramatically increases during sporulation of E. stiedai and E. vermiformis. Partial characterization of the acid phosphatase activity of E. vermiformis indicates that sporozoites account for greater than 92% of the total activity in sporubted oocysts, that the enzyme is resistant to inhibition by tartrate, and that it can be separated into two forms by anion exchange chroma‐tography.