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Superiority of Hemoglobin to Hemin for Cultivation of Leishmania tropica Promastigotes in Serum‐Free Media 1
Author(s) -
GHOLAMHOSSEINIAN A.,
VASSEF A.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04128.x
Subject(s) - hemin , leishmania tropica , hemoglobin , leishmania , microbiology and biotechnology , biology , doubling time , chemistry , biochemistry , leishmaniasis , cell , cutaneous leishmaniasis , immunology , heme , enzyme , parasite hosting , world wide web , computer science
ABSTRACT Leishmania tropica promastigotes grew slowly but could be maintained for long periods in serum‐free hemin‐containing media formulated previously for other Leishmania species or in slightly simplified versions of these media. Replacement of hemin in the medium by hemoglobin resulted in a much longer log phase and a significant reduction in the doubling time. Cell counts in cultures started at 1 times 10 5 cells/ml increased 400‐fold in less than 140 h in the hemoglobin‐containing media. These media also proved suitable for growing L. donovani and L. enriettii promastigotes.