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Characterization of DNA from the Dinoflagellate Woloszynskia bostoniensis 1
Author(s) -
DAVIES W.,
JAKOBSEN K. S.,
NORDBY ø.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04120.x
Subject(s) - dinoflagellate , dna , thymine , kinetics , centrifugation , chemistry , base (topology) , microbiology and biotechnology , biology , biochemistry , botany , physics , mathematical analysis , mathematics , quantum mechanics
Some physical and chemical properties of DNA isolated from the dinoflagellate Woloszynskia bostoniensis were determined. Analytical cesium chloride gradient centrifugation gave a major component and a minor component banding at 1.719 and 1.693 g/cm, respectively. Thermal denaturation in 0.1 SSC showed a broad transition with a T m of 70.5° C. Derivation of this curve indicated that two components were present having T m values of 66° C and 70° C. Base composition analysis showed a GC content of 48.1% and a high degree of thymine replacement by 5‐hydroxymethyluracil. Two minor bases, identified as 5‐methylcytosine and N 6 ‐methyladenine, were also detected. Reassociation kinetics showed a typical eukaryotic reassociation pattern with 45% repetitive and 55% single copy sequences.