Euplotes  Cytoskeleton: Tubulin and Microtubular Systems in Interphase  1 ,  2 | Zendy

DELGADO PILAR | Zendy; CALVO PURIFICACION | Zendy; TORRES ANTONIO | Zendy

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Euplotes Cytoskeleton: Tubulin and Microtubular Systems in Interphase 1 , 2

Author(s) -

DELGADO PILAR,

CALVO PURIFICACION,

TORRES ANTONIO

Publication year - 1988

Publication title -

the journal of protozoology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.067

H-Index - 77

eISSN - 1550-7408

pISSN - 0022-3921

DOI - 10.1111/j.1550-7408.1988.tb04116.x

Subject(s) - tubulin , cytoskeleton , microtubule , tetrahymena , biology , antiserum , cytoplasm , protozoa , macronucleus , microbiology and biotechnology , ciliate , antigen , biochemistry , cell , genetics

The Euplotes cytoskeleton obtained by treatment with Triton X‐100 in a microtubule‐stabilizing buffer has been studied by electron microscopy and SDS‐polyacrylamide electrophoresis. An antiserum has been prepared using the tubulin band from preparative gels of Euplotes cytoskeletons as an antigen. This antiserum reacts with the tubulin from different ciliated protozoa but fails to recognize the vertebrate tubulin by immunoblotting. Immunoblotting studies have demonstrated a slower electrophoretic mobility for the α‐tubulin of Euplotes and Oxytricha than that of Paramecium and Tetrahymena . A cytoplasmic microtubular network in Euplotes has been revealed by indirect immunoftuorescence using both an anti‐α‐tubulin monoclonal antibody directed against chick brain tubulin and an antiserum raised against the tubulin of Euplotes .

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