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Ribosome‐Inactivating Proteins from Plants Inhibit Ribosome Activity of Trypanosoma and Leishmania 1
Author(s) -
CENINI PIETRO,
BOLOGNESI ANDREA,
STIRPE FIORENZO
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04113.x
Subject(s) - ribosome , ribosome inactivating protein , trypanosoma brucei , ricin , biology , protein biosynthesis , eukaryotic ribosome , biochemistry , leishmania major , translation (biology) , leishmania , rna , messenger rna , toxin , gene , parasite hosting , world wide web , computer science
Ribosomes from Trypanosoma brucei rhodesiense and from Leishmania infantum were isolated and optimal conditions for in vitro translation were established. The effect of ribosome‐inactivating proteins extracted from several plants was then assessed in order to identify those suitable for the preparation of immunotoxins against these organisms. Ribosomes from both species were inactivated by some ribosome‐inactivating proteins (dianthins, saporins, pokeweed antiviral proteins, and the ribosome‐inactivating chain of abrin). The similarity of the effects on the ribosomes from the two species examined indicates that ribosome‐inactivating proteins should also be effective in a similar way on ribosomes from other species of Trypanosoma and Leishmania.