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Intranasal Immunization of Mice Against Naegleria fowleri 1
Author(s) -
BUSH LINDA E.,
JOHN DAVID T.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04099.x
Subject(s) - naegleria fowleri , nasal administration , microbiology and biotechnology , virulence , immunization , virology , immunogen , biology , naegleria , meningoencephalitis , lethal dose , inoculation , antibody , immunology , protozoa , monoclonal antibody , toxicology , biochemistry , gene
. The purpose of this research was to determine whether mice could be protected from lethal challenge with Naegleria fowleri by prior intranasal exposure to pathogenic and nonpathogenic Naegleria . Mortality ranged from 0 to 100% for mice inoculated intranasally (i.n.) with 5 × 10 3 amebae of 13 human isolates of N. fowleri . Mice were immunized and challenged i.n. using live amebae of strains of low, medium, and high virulence. The greatest protection against lethal challenge was afforded by three immunizing doses of 10 3 amebae per dose of the strain of medium virulence. Nonpathogenic N. gruberi also was used to immunize mice i.n. against lethal challenge with N. fowleri . Protection was greater following immunization with N. gruberi than it was after immunization with N. fowleri , suggesting that nonpathogenic N. gruberi may be a better immunogen in protecting mice against lethal naeglerial challenge.