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Long Term Cryopreservation of the Amastigote Stages of Hemoflagellates
Author(s) -
YAEGER ROBERT G.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04088.x
Subject(s) - amastigote , cryoprotectant , trypanosoma cruzi , cryopreservation , leishmania donovani , biology , leishmania tropica , strain (injury) , microbiology and biotechnology , leishmania , visceral leishmaniasis , immunology , parasite hosting , anatomy , leishmaniasis , embryo , cutaneous leishmaniasis , world wide web , computer science
. Tissue homogenates containing amastigotes of either Leishmania donovani, L. tropica , or Trypanosoma cruzi were rapidly frozen with 10% glycerol as cryoprotectant. Viability and pathogenicity were maintained for at least 23 years with the Khartoum strain of L. donovani , 22 years with the Malakal strain of L. donovani , and 7 years for L. tropica and T. cruzi . Similar results over a shorter period of time were obtained with a slow‐freezing technique.