Premium
Fluorescence‐Activated Cell‐Sorting Analysis of Fibronectin Peptides Binding to Trypanosoma cruzi Trypomastigotes 1
Author(s) -
OUAISSI M. ALI,
KUSNIERZ J. P.,
GRASMASSE H.,
DROBECQ H.,
VELGE P.,
CORNETTE J.,
CAPRON A.,
TARTAR A.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04087.x
Subject(s) - cell sorting , trypanosoma cruzi , fibronectin , peptide , receptor , biology , microbiology and biotechnology , cell , biochemistry , peptide sequence , parasite hosting , gene , world wide web , computer science
. The binding of synthetic peptides modeled from the sequence of the cell attachment site of fibronectin to T. cruzi trypomastigote surface receptors was investigated by fluorescence‐activated cell‐sorting analysis using fluorescein‐labeled peptides. Peptides with the sequence Arg‐Gly‐Asp‐Ser bound to the parasite surface. A low percentage of fresh parasites recently liberated from infected fibroblasts had the capacity to bind the peptide. In contrast, these parasites showed a time‐dependent several‐fold increase in their ability to bind the Arg‐Gly‐Asp‐Ser‐containing peptides during extracellular incubation. From these observations, it appears that the expression of surface receptors on a particular, mature stage of the parasite parallels its ability to adhere to and infect host cells.