Premium
Evidence of Extensive Fragmentation of Macronuclear DNA in Two Non‐Hypotrichous Ciliates
Author(s) -
METENIER G.,
HUFSCHMID J.D.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04079.x
Subject(s) - dna , agarose gel electrophoresis , fragmentation (computing) , dna fragmentation , biology , microbiology and biotechnology , agarose , gene , gel electrophoresis , genetics , ecology , apoptosis , programmed cell death
. Total cellular DNA from the ciliates Halteria grandinella and Trithigmostoma cucullulus was analyzed by agarose gel electrophoresis. The macronuclear DNA (MAC DNA) of Halteria consisted of very small fragments, which suggests that the MAC DNA organization of oligotrichs resembles that of hypotrichs (gene‐sized DNA). The MAC DNA of Trithigmostoma , a cyrtophorid having a heteromeric MAC, also existed as small fragments, but with a significant fraction (20–30%) comprising larger molecules unresolved by the method used. It is suggested that MAC heteromery is related to the differential localization of two kinds of DNA molecules of different sizes.