Premium
Isolation of a Methanogenic Endosymbiont of the Sapropelic Amoeba Pelomyxa palustris Greeff
Author(s) -
BRUGGEN J. J. A. VAN,
RENS G. L. M. VAN,
GEERTMAN E. J. M.,
ZWART K. B.,
STUMM C. K.,
VOGELS G. D.
Publication year - 1988
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1988.tb04068.x
Subject(s) - amoeba (genus) , biology , isolation (microbiology) , cytoplasm , botany , microbiology and biotechnology , biochemistry
. One of the two methanogenic endosymbionts of the giant sapropelic amoeba Pelomyxa palustris was isolated in pure culture. The cells were slender non‐motile rods (3 × 0.4 μm), sometimes occurring in chains of 3–4 cells. Ultrathin sections revealed a Gram‐positive cell wall and conically pointed ends with mesosome‐like structures in the cytoplasm. The isolate had a generation time of 10 and 12 h during growth on H 2 /CO 2 and formate, respectively. The optimum growth temperature was 40°C and the optimum pH was 7.8. The G+C content of its DNA was found to be 37.7% mole percent. The isolate was identified as Methanobacterium formicicum .