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Electrophoretic Analysis of Isoenzymes in the Identification of Trypanosomatids of the Genus Phytomonas 1
Author(s) -
Vainstein Marilene Henning,
Silva João Batista Tavares,
Lima Vilneide M. Q. Gonçalves,
Roitman Isaac,
Souza Wanderley,
Dollet Michel,
Camargo E. Plessmann
Publication year - 1987
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1987.tb03210.x
Subject(s) - phosphoglucomutase , isozyme , biology , malate dehydrogenase , euphorbiaceae , biochemistry , dehydrogenase , genus , chemotaxonomy , enzyme , botany , microbiology and biotechnology , taxonomy (biology)
Five strains of trypanosomatids of the genus Phytomonas , isolated from different species of Euphorbia {Euphorbia heterophylla, E. characias, E. pinea, E. hyssopifolia) and from Manihot escutenta , were cultured and compared through the electrophoretic mobility of isoenzymes of six enzymes: aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), phosphoglucomutase (EC 2.7.5.1), glucose‐6‐phosphate dehydrogenase (EC 1.1.1.49), glucosephosphate isomerase (EC 5.3.1.9), and malate dehydrogenase (EC 1.1.1.40). The strains could be distinguished from one another by their respective isoenzyme profiles.