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Antigenic Relationship Between Plasmodium falciparum and Babesia bovis : Reactivity with Antibodies to Culture‐Derived Soluble Exoantigens 1
Author(s) -
JAMES MARK A.,
MONTENEGROJAMES SONIA,
FAJFARWHETSTONE CAROL,
MONTEALEGRE FEDERICO,
ERICKSON JAMES,
RISTIC MIODRAG
Publication year - 1987
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1987.tb03184.x
Subject(s) - babesia bovis , plasmodium falciparum , biology , antigen , antibody , virology , cross reactivity , babesia , serology , epitope , babesia bigemina , microbiology and biotechnology , in vitro , parasite hosting , immunology , malaria , cross reactions , biochemistry , world wide web , computer science
Antigenic similarities between Plasmodium and Babesia parasites of the phylum Apicomplexa have been previously demonstrated primarily by the serological cross reactivity observed in the indirect fluorescent antibody (IFA) test. We have now studied the antigenic relationship between the human malaria parasite, Plasmodium falciparum , and the hemoparasitic agent of cattle, Babesia bovis , using rabbit monospecific antibodies produced against individual culture‐derived P. falciparum polypeptides and bovine polyspecific antibodies to B. bovis exoantigens. These respective antibodies were found to be distinctly cross reactive in the IFA test using infected erythrocytes (squirrel monkey— P. falciparum ; bovine— B. bovis ) as antigen substrates. Immunofluorescence was shown to be highly specific for parasite surfaces. Additionally, the degree of reactivity with soluble exoantigens contained in Plasmodium and Babesia culture supernatants was monitored by a two‐site enzyme immunoassay employing the cross‐reactive antibodies. Further evidence for antigenic cross reactivity between P. falciparum and B. bovis parasites was shown with the in vitro inhibition assay. Antibodies to P. falciparum and B. bovis were found to be highly inhibitory for the in vitro growth of P. falciparum in human erythrocytes.