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Serology of Naegleria fowleri and Naegleria lovaniensis in a Hospital Survey 1
Author(s) -
DUBRAY BARBARA L.,
WILHELM WALTER E.,
JENNINGS B. R.
Publication year - 1987
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1987.tb03183.x
Subject(s) - naegleria fowleri , serology , naegleria , antibody , titer , biology , immunoperoxidase , virology , microbiology and biotechnology , antibody titer , immunology , meningoencephalitis , protozoa , monoclonal antibody
An avidin‐biotin horseradish peroxidase method was used to detect antibodies to Naegleria fowleri and N. lovaniensis in human serum samples. Antibodies were detected in 101 specimens from 115 hospital patients ranging in age from 15 to 98 years. Class‐specific anti‐immunoglobulins identified antibodies as IgG and IgM. IgG antibody titers to both species ranged from 1:20 to 1:640. Seven of 15 serum samples collected from newborn infants also demonstrated IgG antibodies to these organisms with a titer range of 1:20 to 1:80. The immunoperoxidase test and Western blot analysis of selected serum samples demonstrated a close similarity in serological results between N. fowleri and N. lovaniensis .