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Ultrastructural Visualization of Lipids in Trypanosomatids 1
Author(s) -
SOARES MAURÍLIO JOSÉ,
SOUZA MARCOS FARINA,
SOUZA WANDERLEY
Publication year - 1987
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1987.tb03160.x
Subject(s) - ultrastructure , visualization , computer science , biology , computational biology , artificial intelligence , anatomy
An imidazole‐buffered osmium tetroxide solution was used to visualize lipids at the ultrastructural level in the following members of the family Trypanosomatidae: Trypanosoma cruzi, T. dionisii, T. vespertilionis, T. rangeli, Crithidia deanei, C. fasciculata, C. oncopelti , and Blastocrithidia culicis . Electron‐dense material was seen in various lipid droplets found in all parasites and in the multivesicular structure of members of the sub‐genus Schizotrypanum . High contrast of some membranes, mainly those which enclose the mitochondrion, the nucleus, and the endoplasmic reticulum, was observed even in unstained sections. X‐ray microanalysis confirmed that the electron density of lipid droplets of B. culicis and membrane‐bounded dense granules of C. oncopelti was due to the presence of osmium.