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Effects of Iron‐, Manganese‐, or Magnesium‐Deficiency on the Growth and Morphology of Euglena gracilis 1
Author(s) -
HILT KENNETH L.,
GORDON PHILIP R.,
HEIN ANN,
CAULFIELD JOHN P.,
FALCHUK KENNETH H.
Publication year - 1987
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1987.tb03159.x
Subject(s) - euglena gracilis , manganese , magnesium , morphology (biology) , chemistry , biology , zoology , biochemistry , gene , organic chemistry , chloroplast
Iron‐, manganese‐, or magnesium‐deficiency has been induced in Euglena gracilis . Each arrests cell proliferation, decreases the intracellular content of the deficient metal, and increases that of several other metals. Light and electron microscopy of stationary phase cells reveal that Fe‐deficient (‐Fe) cells are similar in size and shape to control organisms. Magnesium‐deficient (‐Mg) cells, however, are larger, and approximately 14% are multilobed, containing 2 to 12 lobes of equal size emanating from a central region. Individual (‐Mg) cells and each lobe of multilobed cells contain a single nucleus. Manganese‐deficient (‐Mn) organisms are morphologically more heterogeneous than (‐Fe) or (‐Mg) cells. Most are spherical and larger than controls. Approximately 15% are multilobed but, unlike (‐Mg) cells, contain lobes of unequal size with either zero, one, or several nuclei present in each. Nuclei of (‐Mn) cells differ in size and shape from those of control, (‐Fe), or (‐Mg) cells. All three deficient cell types accumulate large quantities of paramylon. Other cytoplasmic structures, however, appear normal. Addition of Fe, Mn, or Mg to the respective deficient stationary phase cultures reverses growth arrest and restores normal morphology. The results suggest that Fe‐, Mn‐, and Mg‐deficiencies affect different stages of the E. gracilis cell cycle.