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Growth of Paramecium tetraurelia in Bacterized, Monoxenic Cultures 1
Author(s) -
ENRIGHT WILLIAM J.,
HENNESSEY TODD M.
Publication year - 1987
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1987.tb03149.x
Subject(s) - paramecium , mutant , biology , enterobacter aerogenes , stationary phase , chemistry , microbiology and biotechnology , biochemistry , escherichia coli , chromatography , gene
Wild type and mutant Paramecium tetraurelia were grown in monoxenic cultures by first growing Enterobacter aerogenes on a defined medium and then adding the Paramecium to the stationary phase bacterial culture. The bacterial growth was proportional to the concentration of the carbon source (citrate), and the Paramecium growth was dependent upon both the bacterial density and the starting density of Paramecium . The behavior, electrophysiological properties, ciliary lipid composition, and growth characteristics were similar to the commonly used bacterized medium (Cerophyl) except that 5–10 times greater Paramecium yields were reliably obtained.