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Evidence for Phosphorylation of the Extracellular Acid Phosphatase of Leishmania donovani 1
Author(s) -
LOVELACE JAMES K.,
GOTTLIEB MICHAEL
Publication year - 1987
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1987.tb03136.x
Subject(s) - extracellular , leishmania donovani , leishmania , phosphatase , phosphorylation , biochemistry , acid phosphatase , polyacrylamide gel electrophoresis , phosphate , biology , alkaline phosphatase , chemistry , enzyme , microbiology and biotechnology , parasite hosting , world wide web , computer science
The presence of two phosphorylated molecular species in the culture supernatants of axenically cultivated Leishmania donovani promastigotes was demonstrated by biosynthetically labeling cultures with [ 32 P]phosphate. One of these species was resolved into two bands with M r 's of 149,000 and 97,000 by dissociating polyacrylamide gel electrophoresis and copurified with the extracellular acid phosphatase activity produced by the promastigotes. The site of phosphorylation of the extracellular acid phosphatase is not yet known.