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Constitutive and Induced Excretion of Polypeptides in Tetrahymena thermophila 1
Author(s) -
SUHRJESSEN PETER
Publication year - 1987
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1987.tb03131.x
Subject(s) - tetrahymena , excretion , staining , starvation , biochemistry , chemistry , biology , polyacrylamide gel electrophoresis , microbiology and biotechnology , endocrinology , enzyme , genetics
Polypeptides normally excreted to growth or starvation media were revealed using O'Farrell gel‐electrophoresis and silver‐staining. The major polypeptides detected in conditioned media were either constitutive, growth specific, starvation specific, or high‐Tris specific. The majority of the excreted polypeptides could be released from the cells by a hypo‐osomotic shock, possibly resulting in membrane leakage, but also mucocyst and/or plasma membrane‐associated polypeptides were detected in the conditioned media.