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Further Studies on the Organization of the Paraxial Rod of Trypanosomatids 1
Author(s) -
FARINA M.,
ATTIAS M.,
SOUTOPADRON T.,
SOUZA W.
Publication year - 1986
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1986.tb05661.x
Subject(s) - paraxial approximation , axoneme , glutaraldehyde , crystallography , optics , materials science , physics , biophysics , anatomy , biology , chemistry , beam (structure) , biochemistry , flagellum , chromatography , gene
. The fine structure of the paraxial rod of Phytomonas davidi and Herpetomonas megaseliae was analyzed in thin sections of Triton X‐100‐extracted, tannic acid‐glutaraldehyde‐fixed cells and in replicas of quick‐frozen, freeze‐fractured, deeply etched and rotary shadowed cells. The paraxial rod is formed by a complex array of filaments. Two regions, designated as proximal and distal, are formed by two and at least 11 plates, respectively, composed of an association of 25‐nm and 7.0‐nm‐thick filaments which are oriented at an angle of‐50° in relation to the major axis of the axoneme. The intermediate region is less dense and is formed by thin filaments. Short single and Y‐shaped filaments connect the proximal plate to doublets numbers 4 and 7 of the axoneme. Based on the images obtained in stereopairs as well as in photographs obtained before and after inclination of the specimen, a three‐dimensional model of the paraxial rod is proposed.