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Concentration and Enzyme Content of In Vitro‐Cultured Babesia bigemina ‐Infected Erythrocytes 1
Author(s) -
VEGA CARLOS A.,
BUENING GERALD M.,
RODRIGUEZ SERGIO D.,
CARSON C. ANDREW
Publication year - 1986
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1986.tb05653.x
Subject(s) - babesia bigemina , enzyme , lactate dehydrogenase , biology , in vitro , microbiology and biotechnology , biochemistry , parasite hosting , dehydrogenase , glutamate dehydrogenase , enzyme assay , babesiosis , virology , glutamate receptor , receptor , world wide web , computer science
. Clones of in vitro‐cultured Babesia bigemina ‐infected erythrocytes were concentrated by several density gradient procedures. The density range of infected erythrocytes containing pairs of parasites was 1.077 to 1.089 g/ml, whereas the density range of infected erythrocytes containing single parasites was 1.092 to 1.100 g/ml. Three enzymes‐lactate dehydrogenase, glucose‐phosphate isomerase, and glutamate dehydrogenase‐were found associated with infected erythrocytes. The parasite‐specific enzyme and/or isoenzymes were shown to have different mobility patterns in starch gel electrophoresis from those found in the normal bovine erythrocytes. The enzyme 6‐phosphogluconate dehydrogenase was not detected as a parasite‐specific enzyme in B. bigemina ‐infected erythrocytes.