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Determinations of Volumes and Minimal Surface Areas of Individual Compressed Live Tetrahymena 1
Author(s) -
HJELM K. K.
Publication year - 1986
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1986.tb05640.x
Subject(s) - tetrahymena , volume (thermodynamics) , enhanced data rates for gsm evolution , surface (topology) , interference (communication) , materials science , optics , geometry , mathematics , chemistry , physics , computer science , artificial intelligence , telecommunications , thermodynamics , biochemistry , channel (broadcasting)
. A method to determine the volume of individual live Tetrahymena with improved precision has been developed. Cells are compressed between a microslide and coverslip and their volume calculated from their thickness and area determined by interference microscopy and from a photomicrograph, respectively. A formula has been derived to correct for the “edge error” which arises because the rounded edges of the cells are projected on the film as if they were rectangular. The minimal surface area of compressed cells, i.e. that necessary to surround the cells smoothly, can also be calculated. Cells may be recovered after measurements. The method may also be applied to other cells.