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Isolation and Characterization of a Neutral Glycosphingolipid from the Epimastigote Form of Trypanosoma mega 1
Author(s) -
VERMELHO A. B.,
HOGGE L.,
BARRETOBERGTER E.
Publication year - 1986
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1986.tb05591.x
Subject(s) - glycosphingolipid , glycolipid , sphingosine , silicic acid , chromatography , ceramide , fucose , chemistry , sphingolipid , galactose , biochemistry , fatty alcohol , organic chemistry , apoptosis , receptor
. A glycosphingolipid fraction from Trypanosoma mega was isolated after acetylation and was further purified on a silicic acid column. Final purification was by preparative thin‐layer chromatography. The carbohydrate components of the glycolipid were fucose and galactose in approximately equimolar amounts. The neutral glycolipid of T. mega has a sphingosine base composition that consists of sphingosine and traces of dihydrosphingosine. Fatty acids forming amide groups with the sphingosine bases were analyzed by gas‐liquid chromatography‐mass spectrometry and are a mixture of normal and α‐hydroxy fatty acids. Normal C 16:0 , C 18:0 , and 2‐hydroxy C 18:0 are the predominant fatty acids.