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An Improved Procedure for the Purification of Trypanosoma cruzi (Chagas, 1909) Metacyclics from the Insect Vector 1
Author(s) -
STILES BRAD,
KIERSZENBAUM FELIPE
Publication year - 1986
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1986.tb05574.x
Subject(s) - triatoma infestans , trypanosoma cruzi , infectivity , biology , percoll , chagas disease , chromatography , insect , triatominae , centrifugation , virology , chemistry , biochemistry , botany , parasite hosting , virus , world wide web , computer science
. We have developed an improved procedure for isolating and purifying the metacyclic trypomastigote form of Trypanosoma cruzi from infected Triatoma infestans. The procedure was simple, did not require time‐consuming removal of the insect gut, and gave a good recovery of metacyclics. Purification involved centrifugal flotation of the parasites in Percoll followed by diethylaminoethyl cellulose column chromatography. The resulting purified metacyclics exhibited no loss of infectivity when assayed in mice as compared to metacyclics taken directly from the insects.