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Cytoplasmic Inheritance of Temperature Sensitivity in a Wild Stock of Paramecium primaurelia 1
Author(s) -
NYBERG DENNIS
Publication year - 1986
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1986.tb05552.x
Subject(s) - cytoplasm , biology , extranuclear inheritance , phenotype , gene , genetics , paramecium , microbiology and biotechnology , mitochondrial dna
. One stock, GBC, has a maximum temperature of growth about 5°C lower than other recently collected stocks of Paramecium primaurelia. The resistant stocks (R) are able to grow continuously at 35°C while the sensitive stock (S) cells die within 48 h. The F 1 s of R x S crosses exhibited a cytoplasmic pattern of inheritance and all F 2 ‐by‐autogamy lines derived from the S cytoplasmic parent are sensitive. The F 2 ‐by‐autogamy lines cytoplasmically descended from the R parent were predominantly (93%) R in the initial assay. Upon reinvestigation one year later, only 64% of these lines were R, 9% were S, and 27% had a new phenotype, weak (W), intermediate between R and S. Backcrosses of W lines to both R and S strongly suggest that the W lines have normal cytoplasm (i.e. R) but also have nuclear gene(s) for temperature sensitivity that are derived from the original S stock. The delayed manifestation of the W phenotype is not understood.