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Relevance of Hemin for in vitro Differentiation of Trypanosoma cruzi 1
Author(s) -
ARÉVALO JORGE,
PANEBRA ALFREDO,
CRUZ CARLOS SANTA
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb04061.x
Subject(s) - hemin , trypanosoma cruzi , in vitro , biology , chemistry , microbiology and biotechnology , biochemistry , parasite hosting , enzyme , heme , computer science , world wide web
Simple culture conditions that allow good growth and high yields of trypomastigotes are described. The proportion of metacyclic trypomastigotes increases with the concentration of hemin in the culture medium, reaching a peak of 80% after 10 days with 20 mg hemin/liter.