Premium
Updating rDNA Restriction Enzyme Maps of Tetrahymena Reveals Four New Intron‐Containing Species 1
Author(s) -
NIELSEN HENRIK,
SIMON ELLEN M.,
ENGBERG JAN
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb04046.x
Subject(s) - biology , extrachromosomal dna , tetrahymena , tetrahymena pyriformis , restriction enzyme , genetics , restriction map , intron , southern blot , restriction site , restriction fragment length polymorphism , ribosomal rna , microbiology and biotechnology , genome , gene , genotype , nucleic acid sequence
The extrachromosomal rDNA molecules from a number of Tetrahymena strains wered racterized by restriction enzyme mapping using three different restriction enzymes combined with gel blotting and hybridization analysis. Strains from four out of six recently described species were found to contain an intron in the 26s rRNA coding region. The evolutionary relationship among the species of the T. pyriformis complex was examined on the basis of the rDNA maps with emphasis on similarities between two of the new species and the widely studied T. thermophila and T. pigmentosa . Examination of a large number of T. pigmentosa strains showed this species to exhibit an unusual polymorphism with respect to its rDNA. It is suggested that recombinational cross‐over events play a role in the formation of new rDNA alleles in this species.