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Development of the Swine Coccidium Eimeria debliecki Douwes, 1921 in Mammalian Cell Cultures 1
Author(s) -
LINDSAY DAVID S.,
BLAGBURN BYRON L.,
CURRENT WILLIAM L.,
ERNST JOHN V.
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb03099.x
Subject(s) - biology , protozoa , budding , coccidia , eimeria , virology , microbiology and biotechnology , parasite hosting , world wide web , computer science
Sporozoites of Eimeria debliecki entered human fetal lung and porcine kidney cells grown in cultures and underwent one merogenous cycle, terminating in the production of second‐generation trophozoites. Sporozoites were intracellular 1 h post‐inoculation (PI) and developed into sporozoite‐shaped meronts at 40 h PI. These meronts, one of which was motile, had from two to ten nuclei. Sporozoite‐shaped meronts then developed into elongate or spheroidal meronts with 10 to 24 nuclei by two days PI. Ten to 26 first‐generation merozoites were formed by budding from the meront surface. Mature first‐generation merozoites were most numerous three days PI. Most meronts had ruptured and released nonmotile merozoites into the culture medium by four days PI. Merozoites that were not released became rounded and developed into second‐generation trophozoites. Refractile bodies were present in all developmental stages. No further development was observed five through eight days PI.