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Subcellular Distribution of Hydrolases in Naegleria fowleri 1
Author(s) -
David M. Lowrey,
John McLaughlin
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb03089.x
Subject(s) - naegleria fowleri , acid phosphatase , cytoplasm , biochemistry , membrane , enzyme , naegleria , lysosome , chemistry , cell membrane , alkaline phosphatase , cell fractionation , biology , cell , microbiology and biotechnology , protozoa , virology , meningoencephalitis
The presence and particle association of various hydrolytic enzymes in Naegleria fowleri has been studied in whole cell extracts of trophozoites in an effort to establish authentic markers for surface membrane and lysosomal components. Evidence from the experiments reported here indicates that in N. fowleri a) acid proteinase, N‐acetylglucosaminidase, and acid phosphatase are associated with cytoplasmic granules closely resembling lysosomes; b) 5'‐nucleotidase is associated with the surface membrane, probably on the external surface; c) aspartate aminotransferase is associated with mitochondria; d) a‐D‐glucosidase and an aminopeptidase have bimodal distributions, activity being associated with both the surface membrane and lysosomal particles.