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Fluorescein‐Conjugated Folate as an Indicator of Specific Folate Binding to Paramecium 1
Author(s) -
HOUTEN J. L.,
SMITH R.,
WYMER J.,
PALMER B.,
DENARO M.
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb03088.x
Subject(s) - fluorescein , fluorescence , paramecium , conjugated system , chemistry , autofluorescence , biochemistry , folate receptor , microbiology and biotechnology , biophysics , biology , genetics , organic chemistry , physics , cancer , cancer cell , polymer , quantum mechanics
Normal Paramecium tetraurelia cells stained with fluorescein‐conjugated folate show intense fluorescence that can be reduced to near background autofluorescence with excess K 2 ‐folate, but not with excess KCl. Mutant d4–534, which is not attracted to folate and does not specifically bind 3 H‐folate, shows reduced fluorescence when stained. This method of monitoring specific folate binding to cells can be adapted to a microscale for rapid screening of clones since cells are routinely fixed and stained in microtiter wells.