Premium
Incorporation and Toxicity of 32P‐Orthophosphate and Occurrence of Poly phosphate in Entamoeba Trophozoites 1
Author(s) -
LópezRevilla Rubén,
GómezDomínguez Rodolfo
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb03065.x
Subject(s) - entamoeba histolytica , axenic , entamoeba , phosphate , polyphosphate , pi , toxicity , population , microbiology and biotechnology , chemistry , cytoplasm , biology , biochemistry , medicine , bacteria , organic chemistry , genetics , environmental health
We explored the requirements of inorganic phosphate (Pi), the incorporation of 32 P‐orthophosphate ( 32 Pi), and the occurrence of inorganic polyphosphate (polyP) in axenic Entamoeba cultures. Maximal population densities and growth rates of Entamoeba histolytica trophozoites were attained in complete TP‐S‐1 medium. As 32 Pi concentration was increased in the medium, its own incorporation and the culture growth rate were progressively inhibited, especially in Pi‐deficient medium. PolyP grains were found in the cytoplasm and occasionally in the nuclear membrane of E. histolytica, E. histolytica‐like, E. invadens , and E. moshkovskii trophozoites.