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Macrophage Activation by Tetrahymena pyriformis. I. Active Subcellular Fractions of Tetrahymena
Author(s) -
Makioka Asao,
Kobayashi Akio
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb03030.x
Subject(s) - tetrahymena pyriformis , macrophage , in vitro , biology , hydrogen peroxide , microsome , stimulation , tetrahymena , subcellular localization , pinocytosis , biochemistry , microbiology and biotechnology , chemistry , endocytosis , cell , cytoplasm , neuroscience
Experiments were carried out to determine what subcellular fractions of Tetrahymena pyriformis could, after inoculation into mice, activate macrophages to kill Toxoplasma gondii in vitro. Peritoneal macrophages from mice inoculated intraperitoneally with cilia, pellicles, mitochondria, and microsomes exhibited strong toxoplasmacidal activity and had an enhanced capacity to release hydrogen peroxide (H 2 O 2 ) by stimulation of a membrane‐active agent as compared with resident macrophages. In contrast, macrophages from mice inoculated with macronuclei and postmicrosomal supernatant showed no toxoplasmacidal activity and a low level of H 2 O 2 release. Similar dose response was observed on the active subcellular fractions with regard to the degree of macrophage activation. Treatment of the active subcellular fractions with heating and trypsin markedly reduced their activity.