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Purification of Tissue Forms (Amastigotes) of Trypanosoma cruzi by Immunoaffinity Chromatography 1
Author(s) -
CamposNeto Antonio,
Lima Fernanda W. M.,
Andrade Arnaldo F. B.
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb03018.x
Subject(s) - trypanosoma cruzi , amastigote , affinity chromatography , glycolipid , mannose , glycoprotein , biochemistry , antiserum , biology , chromatography , chemistry , microbiology and biotechnology , parasite hosting , antigen , leishmania , immunology , enzyme , world wide web , computer science
A rapid and simple method for the purification of amastigotes of Trypanosoma cruzi from spleens of infected mice is described. A protein A‐Scpharose 4B immunoadsorbent column bound with antisera to epimastigotes of T. cruzi was used to purify the tissue forms of this parasite. Host cells and debris are not retained, and parasites can be eluted in high yields and purity. Studies of surface glycoproteins and glycolipids of the purified amastigotes with 18 lectins of various specificities revealed the presence on the parasites of receptors for N ‐acetylglucosamine, N ‐acetylgalactosamine, D‐galactose, and D‐mannose binding lectins.