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Trypanosoma cruzi : Biological Characterization of Clones Derived from Chronic Chagasic Patients. II. Quantitative Analysis of the Intracellular Cycle 1
Author(s) -
Engel Juan C.,
Doyle Patricia S.,
Dvorak James A.
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb03017.x
Subject(s) - trypanosoma cruzi , amastigote , intracellular , chagas disease , biology , doubling time , population , parasite hosting , virology , microbiology and biotechnology , genetics , cell culture , leishmania , world wide web , computer science , demography , sociology
The complete intracellular cycle of five cloned stocks of Trypanosoma cruzi was quantified. Marked but stable interclonal differences were found in the length of the pre‐replicative lag period (18.2‐34.2 h), amastigote doubling lime (8.6‐21.5 h), and duration of the complete intracellular cycle (96‐215 h). Strong correlations were demonstrated between these characteristics as well as to the growth rate of the epimastigote stage of the same clones grown in liquid medium. These data demonstrate that the marked heterogeneity of the natural population of T. cruzi extends to the intracellular cycle of the parasite and has important implications for our understanding of Chagas’ disease.