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Effects of Long‐Term in Vitro Cultivation on Leishmania donovani Promastigotes
Author(s) -
Nolan Thomas John,
Herman Robert
Publication year - 1985
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1985.tb03015.x
Subject(s) - infectivity , leishmania donovani , hamster , virulence , amastigote , biology , in vitro , leishmania , microbiology and biotechnology , macrophage , catalase , leishmaniasis , virology , immunology , visceral leishmaniasis , biochemistry , oxidative stress , parasite hosting , gene , virus , world wide web , computer science
Promastigotes of Leishmania donovani that had been subcultued in modified Tobie's medium for 2 to 3 years showed decreased infectivity and lack of virulence for hamsters and mice compared to newly transformed promastigotes. Amastigotes derived from these long‐term promastigote cultures decreased in number rapidly in hamsters, but only slightly in mice, over a 48‐day period. In cultured mouse and hamster macrophags infected in vitro, amastigotes derived from long‐term cultures rapidly decreased to low numbers, which persisted. The same pattern was seen in macriphages treated with catalase, an inhibitor of the oxygen‐dependent killing mechanism of the macrophage. Promastigotes from long‐term cultures also differed from virulent first‐passage promastigotes in size, growth patterns in Tabie's medium, and in the quantities of some of their antigens.