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Development of Second Generation Merozoites of Leucocytozoon caulleryi In Vitro
Author(s) -
ISOBE T.,
YAMAGUCHI S.,
IMADA T.,
KAWAKITA F.,
AKIBA K.
Publication year - 1984
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1984.tb04316.x
Subject(s) - petri dish , incubation , inoculation , biology , gametocyte , in vitro , leucocytozoon , incubation period , parasite hosting , microbiology and biotechnology , andrology , immunology , biochemistry , plasmodium falciparum , malaria , medicine , world wide web , computer science
Sporozoites of Leucocytozoon caulleryi were inoculated into specific‐pathogen‐free (SPF) chickens intravenously. Fourteen days after inoculation, the infected blood, parasitized with second generation merozoites, was collected from the chickens. The blood was then suspended in SPF chicken serum or RPMI‐1640 culture medium supplemented with 20% SPF chicken serum in petri dishes, which were cultured at 37°C or 41°C in a humidified atmosphere of 5% CO 2 . Second generation merozoites in parasitized, immature erythrocytes developed continuously through several substages and finally became micro‐ and macro‐gametocytes after 6 days of incubation.