Premium
Purification of Eimeria Sporozoites by DE‐52 Anion Exchange Chromatography
Author(s) -
SCHMATZ DENNIS M.,
CRANE MARK ST. J.,
MURRAY P. KEITH
Publication year - 1984
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1984.tb04314.x
Subject(s) - infectivity , eimeria , chromatography , sodium , ion chromatography , ion exchange , chemistry , chloride , cellulose , biology , microbiology and biotechnology , ion , virology , biochemistry , organic chemistry , virus
An anion exchange column of DE‐52 has been used to purify Eimeria sporozoites from a post‐excystation mixture of oocysts, oocyst shells, sporocysts, sporocyst shells, and sporozoites. The mean recovery from several experiments was 94% and virtually all non‐sporozoite material was removed. Infectivity studies in vitro with sporozoites showed that they were viable after purification and were at least as infectious as the unpurified sporozoites; furthermore, oocysts in the crude preparation could be recovered from the DE‐52 cellulose by resuspending them in a 20% (w/v) sodium chloride solution.