Premium
Absence of α‐Ketoglutarate Dehydrogenase Activity and Presence of CO 2 ‐Fixing Activity in Plasmodium falciparum Grown In Vitro in Human Erythrocytes 1
Author(s) -
BLUM J. J.,
GINSBURG H.
Publication year - 1984
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1984.tb04310.x
Subject(s) - plasmodium falciparum , citric acid cycle , in vitro , biochemistry , bicarbonate , biology , glutamate dehydrogenase , dehydrogenase , lactate dehydrogenase , metabolism , parasite hosting , glutamate receptor , enzyme , microbiology and biotechnology , malaria , receptor , immunology , world wide web , computer science , endocrinology
Plasmodium falciparum ‐infected human erythrocytes grown in vitro do not release 14 CO 2 , when incubated in the presence of [1‐ 14 C]glutamate, despite the presence of glutamate dehydrogenase, implying the absence of α‐ketoglutarate dehydrogenase activity and the lack of functional tricarboxylic acid cycle in the human malaria parasite. Cultures incubated with [ 14 C]bicarbonate, however, fix CO 2 into acid‐stable metabolites; CO 2 fixation proceeds linearly for up to two hours after an initial brief lag and may contribute appreciably to the metabolism of the parasite.