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Surface Anionic Groups in Symbiote‐Bearing and Symbiote‐Free Strains of Crithidia deanei 1
Author(s) -
ODA LEILA M.,
ALVIANO CELUTA S.,
FILHO FERNANDO C. SILVA,
ANGLUSTER JAYME,
ROITMAN ISAAC,
SOUZA WANDERLEY DE
Publication year - 1984
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1984.tb04301.x
Subject(s) - sialic acid , neuraminidase , crithidia fasciculata , thin layer chromatography , chemistry , cytochemistry , biochemistry , electrophoresis , chromatography , dna , enzyme
The surface anionic groups of symbiote‐bearing and symbiote‐free strains of Crithidia deanei were compared by determining cellular electrophoretic mobility, by ultrastructural cytochemistry, and by identification of sialic acids by thin‐layer and gasliquid chromatography. Symbiote‐free Crithidia deanei has a highly negative surface charge (‐0.9984 μmċs ‐1 ċ V ‐1 ċ cm), which is slightly reduced (‐0.8527 μmċs ‐1 ċ V ‐1 ċ cm) by the presence of the endosymbiote. Treatment of both strains of C. deanei with neuraminidase decreased significantly the electrophoretic mobility of cells toward the cathodic pole, indicating the existence of exposed sialic acid residues responsible for the negative charge on the protozoan cell surface. Thin‐layer and gas‐liquid chromatography showed that N ‐glycolyl‐ and N ‐acetylneuraminic acids were present in both strains of C. deanei .