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Localizing Surface‐Related Proteins in Ciliates by Indirect Immunofluorescence Microscopy 1
Author(s) -
WILLIAMS NORMAN E.
Publication year - 1984
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1984.tb03002.x
Subject(s) - immunofluorescence , antiserum , fixation (population genetics) , indirect immunofluorescence , formaldehyde , fluorescence , antigen , chemistry , biology , ethanol , biophysics , biochemistry , antibody , immunology , optics , gene , physics
Results obtained in immunofluorescence localization studies involving three antisera, six species of ciliates, and a variety of fixation procedures suggest that superior results can often be obtained by fixing cells in 35–70% ethanol. Formaldehyde fixation appeared to induce redistributions of epiplasmic proteins and surface antigens which were not observed in ethanol‐fixed cells. In addition, background fluorescence was significantly lower in ethanol‐fixed cells than it was in cells fixed in aldehydes.