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Tandemly Repeated Sequence Families in Micronuclear DNA of the Ciliate Stylonychia pustulata 1
Author(s) -
DAWSON DEAN,
STETLER DEBORAH J.,
SWANTON MARSHAL T.,
HERRICK GLENN
Publication year - 1983
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1983.tb05334.x
Subject(s) - biology , psti , ciliate , repeated sequence , homology (biology) , dna , microbiology and biotechnology , genetics , base pair , tandem repeat , ciliata , nucleic acid thermodynamics , sequence (biology) , gene , base sequence , protozoa , restriction enzyme , genome
One third of a collection of cloned Stylonychia pustulata micronuclear DNA PstI fragments were found to be of a similar size, consistent with their being members of a repetitious sequence family with a repeat size of about 160 base pairs. Cross‐hybridization experiments confirmed that these small cloned fragments are related by sequence homology. Hybridization of the cloned repetitious sequences to PstI digested micronuclear DNA revealed a “ladder” of bands (step size = 160 base pairs), indicating that the repeats are found in tandem arrays. This is the first demonstration of highly repetitious, tandemly repeated sequences in a ciliated protozoan.