Premium
Conjugation Between G 1 and G 2 Phase Cells in Paramecium tetraurelia 1
Author(s) -
MIWA ISOJI,
UMEHARA NORITOSHI
Publication year - 1983
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1983.tb02914.x
Subject(s) - macronucleus , paramecium , micronucleus , cell cycle , meiosis , microbiology and biotechnology , dna , biology , conjugate , mitosis , mating type , genetics , chemistry , cell , micronucleus test , gene , mathematical analysis , mathematics , organic chemistry , toxicity
Cells of Paramecium tetraurelia , stock hr d , cultured in a micro‐capillary containing 1 μl fresh culture medium, expressed mating activity through the whole cell cycle. Mating‐reactive G 2 phase cells can conjugate with cells of other phases. The G 2 phase cells, which have double (4C) the normal micronuclear DNA content, undergo pre‐meiotic DNA synthesis when conjugated with G 1 phase cells. The micronucleus of the progeny from the cross between a G 1 and a G 2 cell becomes triploid.