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Fine‐structural Alterations in Leishmania tropica within Human Macrophages Exposed to Antileishmanial Drugs in Vitro 1
Author(s) -
LANGRETH SUSAN G,
BERMAN JONATHAN D.,
RIORDAN G. PATRICK,
LEE LINDA S.
Publication year - 1983
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1983.tb01421.x
Subject(s) - leishmania tropica , meglumine antimoniate , pentamidine , leishmania , leishmaniasis , in vitro , cutaneous leishmaniasis , macrophage , amastigote , biology , microbiology and biotechnology , chemistry , mechanism of action , immunology , biochemistry , parasite hosting , medicine , world wide web , computer science , pneumonia
The mechanism of action of antileishmanial compounds is poorly understood. Ultrastructural changes in Leishmania tropica within human macrophages exposed in vitro to Pentostam, pentamidine, amphotericin B, WR 6026, ketoconazole, and Formycin B were examined in these experiments. In Pentostam‐treated cultures, some organisms exhibited diminished definition of mitochondrial and other membranes, while other organisms had completely disintegrated. Pentostam‐exposed macrophages demonstrated loss of membrane definition in the absence of further alterations; it is therefore hypothesized that impaired macrophage membrane function may contribute towards the effect of this drug against macrophage‐contained organisms. Leishmania parasites in pentamidine‐treated cultures initially demonstrated swollen kinetoplasts and fragmentation of the kinetoplast DNA core. The initial observed effect of the other four drugs on the parasites was cytoplasmic condensation. These ultrastructural studies suggest that all five non‐antimonial drugs may have different mechanisms of action than antimony (Pentostam) against Leishmania .