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Uptake of Ferritin‐labeled Antibodies by Tetrahymena 1
Author(s) -
WILLIAMS NORMAN E.
Publication year - 1983
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1983.tb01417.x
Subject(s) - tetrahymena , ferritin , endocytosis , antibody , chemistry , biology , biochemistry , microbiology and biotechnology , immunology , cell
Experiments with Tetrahymena thermophila using ferritin probes revealed that these cells take up ferritin conjugated to antibodies (not directed against Tetrahymena ) much more readily than they do ferritin or cationized ferritin. The massive and rapid uptake of antibody‐ferritin offers certain advantages for studies of endocytosis and membrane flow in cells of this type, and the method may be applicable to other types of protozoa as well.