Premium
Surface Changes Induced by Immune Serum on Eimeria tenella Sporozoites and Merozoites
Author(s) -
WITLOCK DALE R.,
DANFORTH HARRY D.
Publication year - 1982
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1982.tb05429.x
Subject(s) - incubation , eimeria , immune system , incubation period , lysis , biology , microbiology and biotechnology , chemistry , immunology , biochemistry
. The surface of merozoites and sporozoites of Eimeria tenella was affected by incubation with E. tenella ‐immune chicken serum (ICS). Normal chicken serum (NCS) and heat‐inactivated ICS had no effect on the pellicular surface of either developmental stage. Sporozoites formed surface bulges or swellings after 10 min of incubation with ICS, and by 15 min postincubation, the morphology of the sporozoites was distorted by a surface coating of fibrinous material. Merozoites exposed to ICS were similarly coated, but surface swelling was not as severe. The coating formed rapidly and was seen as early as 5 min postincubation. Sporozoites incubated with heat‐inactivated ICS supplemented with normal chicken serum were coated with a fibrinous material and in some cases lysed. These data indicated that complement must be present for the surface interaction to occur.