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Ribosomal DNA of Leishmania brasiliensis: Number of Ribosomal Copies and Gene Isolation 1
Author(s) -
VILLALBA ELENA,
RAMIREZ JOSÉ LUIS
Publication year - 1982
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1982.tb05428.x
Subject(s) - ribosomal rna , dna , kinetoplast , microbiology and biotechnology , ribosomal dna , biology , gene , genetics , phylogenetics
. The DNA content of the hemoflagellate Leishmania brasiliensis. strain Y, has been determined by colorimetric reactions and found to be nearly 0.226 pg/cell. When this DNA is bound to filters and hybridized with labeled rRNA from the same organism, saturation is reached at 0.47% of the DNA, corresponding to an estimated 160 ribosomal gene copies. When the DNA is sheared and centrifuged to equilibrium in CsCl gradients, two major satellites of the main band (p = 1.712 g/cm 3 ) are observed: a heavy one (1.720 g/cm 3 ), which hybridizes with labeled rRNA, and a light one (1.699 g/cm 3 ) with the electron microscopic characteristics of the kinetoplast DNA network.