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Trypanosoma cruzi : Flow Cytometric Analysis. I. Analysis of Total DNA/Organism by Means of Mithramycin‐Induced Fluorescence 1, 2
Author(s) -
DVORAK JAMES A.,
HALL THOMAS E.,
CRANE MARK ST. J.,
ENGEL JUAN C.,
McDANIEL JAMES P.,
URIEGAS RODOLFO
Publication year - 1982
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1982.tb05427.x
Subject(s) - trypanosoma cruzi , flow cytometry , dna , biology , kinetoplast , microbiology and biotechnology , organism , nuclear dna , nucleus , fluorescence microscope , genetics , fluorescence , gene , parasite hosting , world wide web , computer science , mitochondrial dna , physics , quantum mechanics
. Total DNA/organism was determined by flow cytometry on stocks of 33 single‐cell‐isolate clones and one strain of mithramycin‐stained Trypanosoma cruzi . Interstrain differences in mean total DNA/group of 34% and interclone differences in total DNA/organism of 41% were found. Microspectrofluorometric analyses of the trypomastigote stage of selected clones confirmed the flow cytometry data and indicated that the total DNA/organism differences were due to differences in DNA of both the nucleus and kinetoplast with the nucleus being the major contributing factor. These data imply that the potential for genetic diversity in T. cruzi may be very large.